What is cholesterol Chod PAP?
Vitro Scient – Cholesterol CHOD – PAP (Single Reagent) Cholesterol CHOD – PAP. (Single Reagent) Cholesterol is a steroid with a secondary hydroxyl group in the C3 position, and found in blood, bile, and brain tissue.
What is Chod pod method?
PRINCIPLE OF THE METHOD The enzyme cholesterol oxidase, in the presence of oxygen, oxidizes cholesterol to cholest-4-en-3one and hydrogen peroxide. Hydrogen peroxide oxidizes phenol and 4-aminoantipyrine to produce red color that can be measured spectrophotometrically.
What is the enzymatic method for cholesterol determination?
An enzymatic method is described for determination of total serum cholesterol by use of a single aqueous reagent. The method requires no prior treatment of sample and the calibration curve is linear to 600 mg/dl. Cholesterol esters are hydrolyzed to free cholesterol by cholesterol ester hydrolase (EC 3.1. 1.13).
How do you test cholesterol for a sample?
Cholesterol is measured using a blood test. A healthcare provider will draw your blood using a needle and collect it in a vial. This typically takes place at your doctor’s office or at a lab where the blood is then analyzed. The test only takes a couple of minutes and is relatively painless.
What is the principle of cholesterol test?
The basic principle of the method is as follows. The apoB containing lipoproteins in the specimen are reacted with a blocking reagent that renders them non-reactive with the enzymatic cholesterol reagent under conditions of the assay.
What cholesterol level is normal?
Total cholesterol levels under 200 mg/dl are healthy for adults. Doctors treat readings of 200–239 mg/dl as borderline high, and readings of at least 240 mg/dl as high.
Which of the following methods for HDL cholesterol is the reference method?
Which of the following methods for HDL cholesterol is the reference method? Ultracentrifugation of plasma in a potassium bromide solution with a density of 1.063 is used to separate HDL from LDL and VLDL.