Under what conditions does Indophenol appear blue?

Under what conditions does Indophenol appear blue?

The indophenol method for determining ammonia in water samples is based on the formation of an indophenol blue pigment during the reaction of phenol and hypochlorite in the presence of ammonia. In alkaline medium (pH = 8–11.5) a chloramine is first produced.

What does Indophenol test for the presence of?

Indophenol is used to indicate the presence of vitamin C in a substance by turning from blue to clear. During an experiment, 0.100% vitamin C is dropped into Indophenol which then turns clear.

Under what chemical conditions does Indophenol appear clear?

Under what chemical conditions does Indophenol appear clear? When a liquid containing Vitamin C is added to Indophenol Solution, the colour changes from Blue to Clear. The more drops of liquid it takes to change the colour, the LESS the amount of Vitamin C there is in the liquid.

How is Indophenol formed?

In this method, the ammonia generated is converted into chloramine on reaction with a sodium hypochlorite solution, which then reacts with phenol to produce the blue-colored indophenols.

What is the Colour of indophenol?

deep blue dye
Indophenol is an organic compound with the formula OC6H4NC6H4OH. It is deep blue dye that is the product of the Berthelot’s reaction, a common test for ammonia. The indophenol group, with various substituents in place of OH and various ring substitutions, is found in many dyes used in hair coloring and textiles.

What does Dcpip stand for?

2,6-Dichlorophenolindophenol (DCPIP, DCIP or DPIP) is a chemical compound used as a redox dye. When oxidized, DCPIP is blue with a maximal absorption at 600 nm; when reduced, DCPIP is colorless.

How do you make indophenol solution?

Fun Fact: You can make an indophenol solution by mixing 0.5 g of indophenol powder with 1 cup of water.

How would you test for the presence of vitamin C in juice?

Place about 10 drops (a small squirt will do) of starch solution into the juice, using the dropper bottle labeled “starch.” Using the dropper pipette labeled “iodine,” add the iodine solution drop by drop (dropwise) into the well until a blue-black precipitate is formed, being sure to count the number of drops.

What is the color of indophenol?

Which of the following method for ammonia measurement is based on the production of indophenol blue from hypochlorite and sodium nitroprusside?

It is freed by the Kjeldahl method from organic matter and can be spectrophotometrically determined by reaction with phenol and hypochlorite in the presence of nitroprusside ion, with which it yields a blue compound (indophenol) with an absorbance maximum at 625nm.

What color is reduced DPIP?

When reduced, however, it turns colorless. Since DPIP replaces NADPH in the light reactions, it will turn from blue to colorless when reduced during photosynthesis.

Why is DCPIP a good indicator?

DCPIP can also be used as an indicator for vitamin C. If vitamin C, which is a good reducing agent, is present, the blue dye, which turns pink in acid conditions, is reduced to a colorless compound by ascorbic acid.