What is the formula for calculating hematocrit?

What is the formula for calculating hematocrit?

Hematocrit is the packed spun volume of whole blood that is made up of RBCs and is expressed as a percentage of total blood volume. It can be measured or calculated as Hct = (RBC × MCV)/10.

What are the components of a hematocrit test?

A hematocrit test is a type of blood test. Your blood is made up of red blood cells, white blood cells, and platelets. These cells and platelets are suspended in a liquid called plasma. A hematocrit test measures how much of your blood is made up of red blood cells.

What is the composition of hematocrit?

Hematocrit (HCT) is the calculated volume percentage of red blood cells (erythrocytes) in your blood. Hematocrit is also called packed cell volume (PCV) or erythrocyte volume fraction. Human blood contains red blood cells, white blood cells, and platelets suspended in a liquid called plasma.

What is the principle of hematocrit test?

PRINCIPLE: When anticoagulated whole blood is centrifuged, the space occupied by the packed red blood cells is defined as the hematocrit (HCT), and is expressed as the percent of red blood cells in a volume of whole blood.

How do you calculate hematocrit from hemoglobin?

The ratio of hematocrit to hemoglobin in healthy people is typically three to one. On this assumption, if you’ve only had your hemoglobin measured, you can estimate the hematocrit by multiplying it by 3. You can also convert the value of hematocrit to hemoglobin by dividing it by 3.

How do you calculate hematocrit from PCV?

Spun HCT and Spun PCV are different names for the same thing (both are derived by the same method—determined by centrifugation of anticoagulated whole blood). Calculated HCT is determined by automated hematology instruments (impedance cell counters). It calculates HCT using the following formula: MCV x RBC count/10.

Is hematocrit and hemoglobin the same?

Hemoglobin and hematocrit are both red blood cell measurements that are used to help diagnose nutritional deficiencies, acute illnesses, and chronic medical conditions. Hemoglobin is a protein in the red blood cells (RBCs), and hematocrit reflects the percentage of blood volume that is composed of red blood cells.

Which combination of reagents is used in measuring hemoglobin?

Drabkin’s Reagent is used for the quantitative, colorimetric determination of hemoglobin concentration in whole blood at 540 nm. Drabkin’s Solution reacts with all forms of hemoglobin except sulfhemoglobin, a pigment that normally occurs in only minute concentrations in blood.

What are the different methods of hematocrit determination?


  • The determination of hematocrit by means of centrifugation.
  • The calculation of hematocrit from the complete blood cell count (CBC)
  • The determination of hematocrit by conductivity.
  • The calculation of hematocrit from ctHb.

What is the rule of 3 in hematology?

What is the rule of three? All abnormal cell counts should be repeated two more times to verify the results. The RBC count is generally three times greater than the hemoglobin. If red blood cells are normochromic and normocytic, the hemoglobin times 3 will approximate the HCT.

Is hematocrit measured or calculated?

With modern lab equipment, the hematocrit can be calculated by an automated analyzer or directly measured, depending on the analyzer manufacturer. Calculated hematocrit is determined by multiplying the red cell count by the mean cell volume.

How do you calculate hematocrit in Haemoglobin?

Hematocrit, abbreviated Hct, is defined as the percentage of a person’s blood occupied by red blood cells (RBCs). It is calculated as follows: RBC Volume/ Total Blood Sample Volume x 100. The normal Hct value for adult females ranges from 35-45%. Hct in adult males ranges from 40-50%.

How to calculate hematocrit level?

Hematocrit level measurement – Note: Microhematocrit tube after sedimentation. The hematocrit is a ratio of the packed cells to total volume. Example: If the column of packed red cells measures 20 mm and the whole blood column measures 50 mm, the hematocrit is 20/50 = 0.4 or (0.4 × 100%) = 40%.

What are the normal values for hematocrit?

The hematocrit measures the volume of red blood cells compared to the total blood volume (red blood cells and plasma). The normal hematocrit for men is 40 to 54%; for women it is 36 to 48%. This value can be determined directly by microhematocrit centrifugation or calculated indirectly.

How high is too high hematocrit?

When the hemoglobin count is higher than normal, it may be a sign of a health problem. Normal hemoglobin counts are 14 to 17 gm/dL (grams per deciliter) for men and 12 to 15 gm/dL for women. Hemoglobin levels depend on many factors, including age, race, gender and the general health of the person.