What is the role of HIF-1?

What is the role of HIF-1?

HIF-1 is a major regulator of oxygen homeostasis within cells. As a transcription factor, it affects and regulates the expression of dozens of genes involved in maintaining homeostasis as oxygen concentrations change [13].

Is HIF1a tumor suppressor?

Mechanistically, HIF1a represses the aspartate-producing enzymes GOT1 and GOT2 in several biological settings, including human VHL-deficient renal cell carcinoma, in which HIF1a can act as a tumor suppressor.

What does HIF-1 stand for?

Hypoxia-inducible factor 1-alpha
Hypoxia-inducible factor 1-alpha, also known as HIF-1-alpha, is a subunit of a heterodimeric transcription factor hypoxia-inducible factor 1 (HIF-1) that is encoded by the HIF1A gene. The Nobel Prize in Physiology or Medicine 2019 was awarded for the discovery of HIF.

Who discovered HIF-1?

HIF-1 was initially discovered by Semenza and co-workers15 in 1991 during a study conducted on erythropoietin (EPO) gene, a gene responsible for encoding erythropoietin hormone for red blood cells production. Cis-acting DNA sequences (5′–RCGTG-3′) were identified in the 3′-flanking region of this EPO gene.

What is the role of HIF 1alpha in tumor progression?

Activated HIF-1 plays a crucial role in adaptive responses of the tumor cells to changes in oxygen through transcriptional activation of over 100 downstream genes which regulate vital biological processes required for tumor survival and progression.

How do you measure HIF 1a?

It is possible to measure HIF-1α in biological samples using an ELISA assay, although careful sample preparation and storage is essential. This may be of value in conditions in which tissue hypoxia may play a role, including cancer, cardiovascular disease and pulmonary disease.

Is HIF-1 alpha an oncogene?

Clinical data linking HIF-1α and HIF-2α levels to cancer mortality. Taken together, the observed effects of tumor suppressor LOF and transforming virus protein expression provide compelling evidence that HIF-1 activation promotes oncogenesis and/or cancer progression.

How does HIF regulate transcription?

Upon hypoxia, the HIF alpha subunits are stabilized and accumulate in the nucleus, where they dimerize with HIF1B, allowing them to bind to DNA and stimulate the transcription of their target genes.