What is a CTL in biotech?
Cellular Technology Limited (CTL) provides contract laboratory services to the pharmaceutical and biotechnology industry, specialising in cell mediated immune monitoring for research, clinical trials, and preclinical studies. Its services are also utilised by government agencies and academic institutions.
How does an elispot work?
How ELISPOT works. ELISPOT is performed using a PVDF or nitrocellulose membrane 96-well plate pre-coated with an antibody specific to the secreted protein. Cells are added to the plate and attach to the coated membrane. Cells are then stimulated and the secreted protein binds to the antibody.
What is Immunospot assay?
The enzyme-linked immunospot (ELISPOT) assay is a sensitive immunologic method measuring cytokine release on a single-cell basis to detect epitope-specific CD8+ T cells. CD8+ T cells are incubated with a putative antigenic peptide presented by MHC-I on antigen presenting cells (APCs).
How much is an ELISpot reader?
What does ELISpot stand for?
The enzyme-linked immunospot (ELISpot) assay is a highly sensitive immunoassay that measures the frequency of cytokine-secreting cells at the single-cell level. In this assay, cells are cultured on a surface coated with a specific capture antibody in the presence or absence of stimuli.
What is the difference between Elisa and ELISpot?
ELISA is a biochemical assay that is used to detect the presence of a protein by directing an antibody against it. ELISpot is a technique that is used to measure the amount of cytokine secreted by a cell.
What is immune monitoring?
Immune monitoring provides insight into a patient’s own immune response over the course of a treatment.
What is an ELISpot reader?
The basic EliSpot/FluoroSpot Reader A simple “one-click switch” between enzymatic and fluorescent mode, without the need of hardware changes, is enough to switch from one mode to another. The “FluoroAID” image overlay technology permits exact detection of cells secreting multiple cytokines.
Which immune cells produce cytokines?
Cytokines are mainly produced by macrophages and lymphocytes, although they can also be produced by polymorphonuclear leukocytes (PMN), endothelial and epithelial cells, adipocytes, and connective tissue. Cytokines are essential to the functions of macrophages.
What types of cells can be used in ELISPOT?
ELISPOT assays are one of the most sensitive and specific techniques available for monitoring T and B cell responses permitting the ex vivo identification of cells actively secreting signaling proteins or antibodies.
How much does an ELISpot Reader cost?