What is PECAM staining?
Background. CD31 or PECAM-1, is a type I integral membrane glycoprotein that is expressed at high levels on endothelial cells. CD31 staining can be used as a marker of endothelial cells. The expression on endothelial cells is concentrated at junctions between adjacent cells.
What is CD31 positive?
CD31-positive macrophages were distinguished from endothelial or tumor cells by correlation with the morphologic features and the immunohistochemical staining pattern of the cells of interest. In no case was CD31 positivity seen in the lesional cells of a nonvascular tumor.
Is CD31 an integrin?
CD31/PECAM-1 is a ligand for alpha v beta 3 integrin involved in adhesion of leukocytes to endothelium.
Do all endothelial cells express CD31?
PECAM-1 (CD31) is a 130 kDa glycoprotein expressed on endothelial cells, platelets and some leukocytes. CD31 is constitutively expressed on endothelial cells, and its expression is not increased by cytokines.
What is CD31 and CD34?
CD34 is one of the Endothelial Cell Differentiation Markers and Endothelial Progenitor Cell Markers. CD31 is normally found on endothelial cells, platelets, macrophages and Kupffer cells, granulocytes, T / NK cells, lymphocytes, megakaryocytes, osteoclasts, neutrophils.
What is the function of CD31?
What is The Function of CD31? As mentioned, CD31 expression is restricted to blood and vascular cells. In circulating platelets and leukocytes, CD31 functions mainly as an inhibitory receptor that, via regulated sequential phosphorylation of its cytoplasmic domain, limits cellular activation responses.
What does P selectin do?
P-selectin mediates the rolling of blood cells on the surface of the endothelium and initiates the attachment of leukocytes circulating in the blood to platelets, endothelial cells,and other leukocytes at sites of tissue injury and inflammation.
Do macrophages express CD31?
Immunofluorescence analysis revealed that green fluorescent protein-positive cells in the walls of new vessels in wounds, gel foam blocks, and tumors expressed both F4/80 and CD31, that is, macrophages.