What is RF Turbidimetry?
Latex Turbidimetry. PRINCIPLE. The latex particles coated with human gammaglobulin are agglutinated when they react with samples that contain rheumatoid factors (RF). The latex particles agglutination is proportional to the concentration of the RF in the sample and can be measured by turbidimetry.
What is RF blood test done for?
A rheumatoid factor test is one of a group of blood tests primarily used to help pinpoint a diagnosis of rheumatoid arthritis. These other tests may include: Anti-nuclear antibody (ANA) Anti-cyclic citrullinated peptide (anti-CCP) antibodies.
What is Immunoturbidimetry test?
Immunoturbidimetry measures the absorbance of the light by the sample, nephelometry measures the light scattered at a fixed angle. The level of analyte is determined by comparison with a calibrator of known concentration.
What is quantitative Immunoturbidimetric method?
A quantitative immunoturbidimetric assay for rheumatoid factors (RF) is described, based on the immunoprecipitation between aggregated human IgG and rheumatoid factors in serum. The resulting turbidity is measured photometrically at 340 nm.
What is the principle of RF test?
Principle of the Rheumatoid Factor test If the rheumatoid factor is present in the patient’s blood it attaches to the IgG coating the latex particles causing clumps. This is called agglutination. Agglutination is considered a positive reaction that indicates the presence of rheumatoid factor at a detectable level.
What is RA test positive?
A positive test means there’s a 97% chance you have RA. If you have anti-CCP antibodies, your rheumatoid arthritis might be more severe. Other conditions you might have: None. This test is used only to look for RA. Erythrocyte sedimentation rate (ESR)
What diseases have a positive rheumatoid factor?
If rheumatoid factor is found in your blood, it may indicate:
- Rheumatoid arthritis.
- Another autoimmune disease, such lupus, Sjogren’s syndrome, juvenile arthritis, or scleroderma.
- An infection, such as mononucleosis or tuberculosis.
- Certain cancers, such as leukemia or multiple myeloma.
What diseases can cause a positive ANA?
Conditions that usually cause a positive ANA test include:
- Systemic lupus erythematosus.
- Sjögren’s syndrome — a disease that causes dry eyes and mouth.
- Scleroderma — a connective tissue disease.
- Rheumatoid arthritis — this causes joint damage, pain, and swelling.
- Polymyositis — a disease that causes muscle weakness.
Why is Immunoturbidimetry important?
Immunoturbidimetry is an important tool in the broad diagnostic field of clinical chemistry. It is used to determine serum proteins not detectable with classical clinical chemistry methods. Immunoturbidimetry uses the classical antigen-antibody reaction.
What is Immunoturbidimetry normal range?
The normal range is usually 11 to 69 ng/ml.
What is particle enhanced Immunoturbidimetric?
Particle-enhanced immunoturbidimetric tests are especially useful if an antigen is present in low concentration. By this approach, microscopic particles enlarge the formed immune complexes, amplify the signal and thereby lead to a significantly increased sensi- tivity of this method.
What is the normal RF range?
Negative levels do not exclude the disease, and positive levels do not guarantee the diagnosis. The normal range of RF is from 0-20 IU/ml. RF above 20 IU/ml is not considered enough to diagnose RA, as there other reasons the RF level may be elevated.