Are all 96-well plates the same size?
Corning 96-well polystyrene microplates have plate dimensions (length x width x height) of 127.76 x 85.48 x 14.22 mm that meet standard ANSI/SBS footprint dimensions for microplates. Corning tissue culture-treated microplates have the same surface treatment used on other Corning culture vessels.
What volume fits in a 96-well plate?
CulturPlate Microplates
| Well format | 24-well | 96-well |
|---|---|---|
| Well volume | 2.39 mL | 400 µL |
| Recommended working volume | 0.5-2.39 mL | 80 µL-350 µL |
| Height (mm) | 18.70 | 14.60 |
| Length (mm) | 127.80 | 127.76 |
What is a 96-well plate used for in chemistry?
96-well microplates are used for antibiotic screens, cell-based assays and screening compounds. The flat bottom shape is ideal for microscopic and optical measurements.
Can you reuse 96-well plates?
These plates are not meant to be reused. In our experience when you proceed to autoclave, and repeat an experiment, the results are not the same, if we make a triplicate of known samples, you can give only one or two reference values.
How long is a 96-well plate?
The length of the 96-well standard microplate is 127.71 mm x the width 85.43 mm and the height is 14.10 mm (shown in fig. 1 and fig. 2 below). The 96-well standard format microplate has 12 columns (1-12) and 8 rows (A-H) for easy identification of the cells.
What are microtiter plates used for?
Microtiter plates are most frequently used for screenings and cultivation of large sample amounts. In order to perform an immunoassay (e. g. ELISA = Enzyme Linked Immuno Sorbent Assay) the bottoms of microtiter plate wells are coated with antibodies to detect specific substances within samples.
How much does a 96 well plate cost?
Plate 200 µL of cell culture (i.e., 10,000–50,000 cells) into the wells of the sterile 96-well cell culture plate. Incubate the cells for 18 hours at 37°C. Stimulate the cells as desired.
How deep is a 96 well plate?
How many cells are in a 96 well plate?
Useful information for various sizes of cell culture dishes and flasks
| Catalog No. | Cells at confluency* | |
|---|---|---|
| Dishes | ||
| 24-well | 142475 | 0.24 x 106 |
| 48-well | 150687 | 0.12 x 106 |
| 96-well | 167008 | 0.04 x 106 |
How do you Sterilise a 96 well plate?
1 – Throw the liquid culture from the plate in a bucket and add bleach to it. After 30min, throw the liquid in the sink and clean the bucket and the sink. 2 – Place the plate in a bucket and add bleach mix to water to completely fill the plate. Wait 30min for complete decontamination of the plate.
Are well plates autoclavable?
Deep-well plates made of PP are autoclavable (121 °C , 2 bar, acc.
