Is uricase and urate oxidase same?
Urate oxidase, or uricase (EC 1.7. 3.3), is a peroxisomal enzyme that catalyzes the oxidation of uric acid to allantoin in most mammals. In humans and certain other primates, however, the enzyme has been lost by some unknown mechanism.
What is uricase enzyme?
Uricase or urate oxidase (EC 1.7. 3.3) is a copper-binding enzyme that catalyses the oxidation of uric acid to 5-hydroxyisourate and hydrogen peroxide (H2O2) (Fig. 14-1).
What is uricase method?
Uricase method is widely used to monitor serum uric acid for laboratory diagnosis of gout, kidney function and hyperuricemia-associated diseases. It is easy to quantify uric acid by its distinctive absorbance at 293 nm or to quantify hydrogen peroxide, one of the uricase products, by peroxidase-coupled analysis.
Why do humans not have uricase?
The researchers found that over time, mutations in the gene that codes for uricase made the enzyme progressively less effective. Finally, about 17 million years ago, apes developed a mutation that causes us not to produce any uricase at all.
Which method is most commonly used for uric acid estimation?
Currently, two methods are widely utilized to quantify uric acid. A colorimetric method depends on the reduction of a chromogen such as sodium tungstate by uric acid to produce a measurable color change. This technique has been commonly employed in automated hospital screening (SMA systems).
How does uricase lower uric acid?
Pegloticase. Uricase is an enzyme that converts poorly soluable urate (uric acid) to the more soluable allantoin (excreted in the urine). Uricase is present in most mammals, and these mammals with uricase do not develop gout.
What is the use of uricase?
Nonrecombinant urate-oxidase (uricase) is used in Europe to prevent severe hyperuricemia induced by chemotherapy in patients with malignancies, as well as in selected patients with treatment-refractory gout.
Which method is most commonly used for uric acid?
Currently, the peroxidase-coupled indirect equilibrium uricase method is still the commonest one for serum uric acid assay in clinic laboratory (Table 1).
What foods contain uricase?
Plants which have high amounts of purines include spinach, peas, lenticels, cauliflowers and beans. Any food containing yeast extract should be avoided [60].
What are the different methods for uric acid determination?
Uric acid as measured in serum by three different uricase (EC 1.7. 3.3) methods (aca, Ektachem, and SMAC) and by the SMAC method with phosphotungstic acid was compared with a candidate Reference Method for uric acid.
Which uric acid method is associated with negative bias caused by reducing agents?
Which uric acid method is associated with negative bias caused by reducing agents? The peroxidase-coupled uricase reaction is the most common method for measuring uric acid in serum or plasma.
What are the different test methods done in determining uric acid?
A uric acid test can be done as a blood test or a urine test. During a blood test, a health care professional will take a blood sample from a vein in your arm, using a small needle.