What is 100 bp DNA Ladder?
Invitrogen 100 bp DNA Ladder is designed for sizing and approximate quantification of double-stranded DNA in the range of 100 bp to 2,000 bp. 100 bp DNA Ladder consists of 13 individual chromatography-purified DNA fragments and has reference bands at 2000, 1500, and 600 bp for easy orientation.
How much 100 bp Ladder to Load?
10 μl
We recommend loading 10 μl (0.5 μg) of Quick-Load 100 bp DNA Ladder per gel lane. In general, it is recommended to load 1µl per mm of gel lane.
What does BP mean in DNA ladder?
base pair
Introduction. DNA molecular weight (mw) standard controls of nucleic acids, also known as DNA ladders, are widely used in molecular biology studies to determine the mw or the base pair (bp) length of nucleic acids. The DNA ladder is also used to quantitatively analyze target DNA fragments.
How do you dilute 100 bp DNA ladder?
The ladder is diluted to a 1:4 solution in water for use (3 parts water for 1 part ladder). To make 100 µl, 75 µl of water are combined with 25 µl of the DNA ladder. Then, 20 µl of 6X loading dye is added and the solution is split into 60 µl aliquots (0.5 ml microcentrifuge tubes) and stored at -20 C.
What is a ladder DNA?
A DNA ladder is a solution of DNA molecules of different lengths used in agarose or acrylamide gel electrophoresis. It is applied as a reference to estimate the size of unknown DNA molecules that were separated based on their mobility in an electrical field through the gel.
What is bp in gel electrophoresis?
The term ‘bp’ in gel electrophoresis stands for base pair. Base pairs refer to a set of complementary nucleotides in DNA.
What does 1kb ladder mean?
1 kb DNA Ladder allows for determining the size of double-stranded DNA from 250 – 10,000 base pairs (bp). The ladder consists of 13 double-stranded, blunt-end fragments, of sizes 250, 500, 750, 1000, 1500, 2000, 2500, 3000, 4000, 5000, 6000, 8000, and 10,000 bp (see Figure 1).
Can I dilute DNA Ladder?
Dilute only 1 µl of DNA Ladder at a time DNA may denature if diluted and stored in dH20. This protocol is recommended for a 5mm wide gel lane. The components of the mixture should be scaled up or down, depending on the width of the lane.